|
Title |
Student Name |
Supervisor |
Abstract |
|
Isolation and identification of phytopathogenic fungi from infected plant parts |
Doaa Aburabee Eman Abu Al-Haija Tala Qutob Aya Abu Khader |
Prof. Raed Alkowni |
Fungal infections provide enormous difficulties to agriculture and the food industry by causing illnesses like rusts, smuts, rots, and mildew, as well as manufacturing hazardous mycotoxins. This study seeks to extract and identify fungal pathogens from infected plant components such as tomato, garlic, loquat, Lamium moschatum Milli, strawberry, and rose. Samples were gathered in 2024 from several locations in Palestine, including Tulkarm and Nablus. Both endophytic and postharvest fungal samples were grown on PDA plates, sub-cultured for viability, and stored in a glycerol-water solution. Identification was accomplished through microscopic examination with lactophenol blue dye and molecular approaches by DNA sequencing. DNA extraction was followed by PCR amplification with ITS1 and ITS4 primers, and the results were examined using agarose gel electrophoresis. The sequences were analyzed by using BLASTn searching program at the NCBI, where several fungal pathogens had been identified. This study intends to improve plant disease management by precisely detecting fungal infections, which will aid in the development of effective biological control measures and contribute to agricultural sustainability. |
|
Isolation and identification of phytopathogenic bacteria in vegetable crops in West Bank (Palestine) |
Tahani Horani Muyassar Zaghlool Rana Hanani Hana Shirri |
Prof. Raed Alkowni |
Leaf samples were gathered from agricultural districts around Palestine. Samples were plated on various media to isolate bacterial colonies. Isolated colonies had various morphologies, indicating different bacterial species. Biochemical assays were performed to identify the isolated microorganisms. The tests included gram staining, oxidase, catalase, motility, spore production, and urease activity. The results revealed the presence of many bacterial species with unique biochemical profiles. DNA Sequencing and Analysis were performed using BLAST to identify bacterial species. Phylogenetic trees were created to better comprehend evolutionary relationships. The morphological and biochemical variety of the isolated bacteria indicates the existence of several phytopathogenic species. Identification and characterization of these microorganisms are critical for building successful regional disease management strategies. This section emphasizes the significance of discovering phytopathogenic bacteria in addressing agricultural difficulties in Palestine and improving crop disease management strategies. |